Batch Fosmid: Small Yet Solid

 by: Paul Jefferson Arnigo (Fosmid)

Fosmids are cloning vectors that can carry large fragments of DNA and are based on the F-factor plasmid. These F-factor replicons are naturally low-copy plasmids, around 1 to 2 copies per cell, reducing the risk of recombination and deletions in large DNA inserts and ensuring the stability of these inserts. Fosmids contain a strictly regulated origin of replication, also known as oriV, and a partitioning system that ensures even distribution to daughter cells. This regulation prevents excessive plasmid replication, reducing the metabolic burden on the host and maintaining genetic stability. Moreover, this mechanism allows the cloning of previously “unclonable” genome segments due to their ability to maintain sequences that are unstable in high-copy vectors.

On a similar template, Batch Fosmid is made up of four unique individuals, with each having their own abilities and sequences. Three students are from BS Agricultural Biotechnology, namely, PJ, Kertz, and Pat, and one student is from BS Nutrition, Jared. Much like how fosmids are DNA stabilizers, each of them aims to serve the organization with utmost integrity, one fragment at a time. Batch Fosmid is a testament that despite being small in number, it can still maintain a solid foundation.

Paul Jefferson “PJ” Arnigo

    Steady, dependable, and rigid, Paul, or “PJ,” handles complex challenges with a calm mind and a sense of humor. He is the kind of person who brings joy and laughter to any room he enters. Beyond his easygoing charm lies a sharp intellect and an instinct for leadership. Whether it's navigating complex challenges or simply offering a thoughtful perspective when it's needed most, he always shows up with a sense of purpose. No matter how daunting the road ahead may seem, there's no doubt he'll face it with grace, courage, and determination. Like fosmids, PJ may be small, but he is also consistent, sincere, and always a step ahead. 

Alezandra Patriz Aragon

    Much like how her name suggests, Patriz, or “Pat,” will support you every step of the way. Whether it may be a lending hand or a simple ‘pat’ on the back, you can surely count on her. Her enthusiastic side blends with her cheerful personality just as she likes her coffee—balanced, never too creamy, never too bland. As a member of the organization, she treats everyone as family, occasionally referring to everyone as “Ate.” Beneath her vibrant smiles is a proud resilience amidst life’s pressures. When it comes to complex puzzles and unexpected interferences, count on Pat that she will always find her path.

Kertzlene “Kertz” Pangilinan

    In the midst of the crowds, Kertz would definitely find you. Her attention to detail, combined with excellent organizational skills, is one of her main assets. Her wits and creative ideas coincide with her fiery passion for excellence. She never fails to light up the room with her positive attitude and a pinch of sarcasm every now and then. Above all, her initiatives and capabilities to lead are part of what makes her the sister figure to the batch. With Kertz, you can be sure that everything will be A-OK.

Jared Von Santiago

    Diligent and accountable—that’s the Jared way. His unique persona will always leave you either laughing or guessing. Just like a book, he is an open-minded fellow, relying on his wits and intellect to connect ideas and solve unexpected plot twists. He values quality over time, ensuring each decision he makes is well-thought-out with utmost consideration. Jared makes time for almost anything, at any place, leaving no corners unchecked and no second wasted. If accountability were a person, Jared would be its name.

SOURCES

Thomsen, P. F., & Willerslev, E. (2015). Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity. Biological conservation, 183, 4-18.

Kim, U. J., Shizuya, H., de Jong, P. J., Birren, B., & Simon, M. I. (1992). Stable propagation of cosmid sized human DNA inserts in an F factor based vector. Nucleic acids research, 20(5), 1083-1085.

Martínez, A., & Osburne, M. S. (2013). Preparation of fosmid libraries and functional metagenomic analysis of microbial community DNA. In Methods in enzymology (Vol. 531, pp. 123-142). Academic Press.

This article was originally published in the GENEWS May 2025 Issue.